Gaulden, a former four-star recruit, broke a bone in his foot at practice Thursday.
The Thompson's Station, Tenn. native played in 11 games last season for the Vols, primarily on special teams, and made seven total tackles.
At Tennessee's Orange and White game this past spring, Gaulden was awarded the Andy Spiva Defensive Player Award. He was expected to start at nickelback this season.
Gaulden's injury is just the latest in the Vols' secondary this preseason, as sophomore cornerback Justin Martin and freshman safety Stephen Griffin have also been sidelined with injuries.
Tennessee opens its season Sept. 5 at home against Bowling Green.
- Kayla Lombardo