The reliever has a strained oblique on his right side and will be put on the DL retroactive to April 28. He'll become the 10th player on New York's disabled list.
"He showed up yesterday at the ballpark early complaining of some mild pain on his right abdomen," Yankees general manager Brian Cashman said Thursday. "When he was in the bullpen, warming up the night before, he felt something."
Chamberlain joins a DL that already included shortstop Derek Jeter (broken ankle), third baseman Alex Rodriguez (hip surgery), first baseman Mark Teixeira (torn tendon sheath in wrist), outfielder Curtis Granderson (broken forearm), infielder Kevin Youkilis (sprained back), catcher Francisco Cervelli (broken hand) and pitchers Ivan Nova (tightness in triceps), Michael Pineda (shoulder surgery) and Cesar Cabral (broken elbow).
"It seems like it's possible it could resolve within the two weeks but I can't certainly guarantee it," Cashman said. "Joba is a pretty miraculous healer, more so than anybody I've seen. He does get hurt, but when he does he usually bounces back pretty quick."
David Robertson, another right-hander reliever, also had a scan Thursday on a knee that has pain where the hamstring attaches. Cashman said Robertson is day to day.
Rodriguez, recovering from hip surgery on Jan. 16, has been running at full speed and has been cleared to start workouts Monday at the team's minor league complex in Tampa, Fla.
"They are now working out a baseball rehab protocol for him," Cashman said.
Rodriguez is expected back after the All-Star break.
Teixeira, injured March 5 while with the U.S. team at the World Baseball Classic, also will report next week to the minor league complex, where Granderson has started hitting in extended spring training.
"We're getting ready to fit the extended spring program with some championship caliber rings," Cashman said.
NOTES: Cashman is recovering from a broken right leg and dislocated ankle, injuries sustained while skydiving on March 4. Speaking at a news conference Thursday to announce a study on opinions of steroids use, former Yankees head athletic trainer Gene Monahan made a reference to the GM. Monahan said conditioning has caused athletes to avoid getting hurt in many instances. "A lot of major injuries now," he said, "are just accidents that happen: Guys getting hit with balls. Guys running into walls. General managers jumping out of airplanes." Monahan may fill in for his successor, Steve Donahue, for a few days next month when Donohue has a family commitment.