It's the first time Wayne has been active since he tore his right ACL in a Week 7 game against the Denver Broncos last season.
Colts coach Chuck Pagano said earlier this week Wayne would be a game-time decision. Wayne said recently he feels ready to play, but the Colts have been cautious with the 35-year-old's recovery process.
Wayne had 38 catches for 503 yards and two touchdowns before tearing his ACL last year. The six-time Pro Bowler is entering his 14th NFL season, all with Indianapolis.
The Colts open the regular season Sept. 7 at the Broncos.
- Ben Estes