Lee left practice early on Wednesday with tightness in his hamstring.
Jacksonville drafted Lee with the No. 39 pick in this year's NFL draft out of USC. The rookie has eight catches for 73 yards through two games.
Jaguars coach Gus Bradley criticized the team's rookie receivers earlier this week for making too many mental errors.
Jacksonville, 0-2, hosts the Colts on Sunday in its home opener.
- Paul Palladino