Lions coach Jim Caldwell said Pettigrew was suspended for an unspecified violation of team rules but refused to go into details.
"It was punishment for something that I did. It's over with," Pettigrew said. "Just a quarter. Back to business."
Pettigrew, who has 10 catches for 70 yards this season, did not catch a pass and was not targeted in the game. He entered the game at the beginning of the second quarter.
The victory over the Buccaneers moved Detroit to 9-4, second place in the NFC North. Detroit takes on the Minnesota Vikings next week.
- Scooby Axson