Amendola was listed as questionable with a knee injury during the week, but he did not make the trip to Denver with the team, according to the reports. The Patriots will also be without receiver Julian Edelman, who has a broken foot.
Amendola has 49 catches for 520 yards and two touchdowns so far this season. He played in all 16 games for the Patriots in 2014.
Linebacker Jamie Collins and tight end Michael Williams were also ruled out for New England.