The quarterback was officially listed as "Did Not Practice" on the team's Friday injury report, despite making some "soft tosses" during portions of Friday's practice.
Locker has not practiced all week, and is expected to be replaced on Sunday by Charlie Whitehurst.
During his time in the NFL, Locker has missed 15 of a potential 37 starts due to various injuries.
Locker, currently in his fourth year in the league, has completed 58 percent of his passes for 764 yards, four touchdowns and four interceptions. His quarterback rating for the season so far is 75.9.
The Titans are 1-4 so far this season, and are currently third in the AFC South.
- Christopher Woody