Rooney's goal came in the 33rd minute, putting D.C. United up 1-0 over the Colorado Rapids.
In only his second start in Major League Soccer, Wayne Rooney has scored his first goal in the league.
Rooney scored in the 33rd minute, as he slid the ball between the legs of his former Manchester United teammate Tim Howard. The goal put D.C. United up 1-0 over the Colorado Rapids. United's Luciano Acosta and Zoltan Stieber were credited with assists.
The forward pumped his fist and jumped in the air to celebrate, as teammates ran over to hug him. The crowd at Audi Field gave Rooney a standing ovation.
Saturday night's match marked only Rooney's fourth MLS appearance and his first as United's new captain.
Later in the match, however, the English forward needed stitches after breaking his nose while defending a corner.
After receiving treatment, he re-entered the game. DC United manager Ben Olsen praised his influence on the team since arriving from Everton last month.
"I don't think it's the first time he's bled in a game. He is a tough guy and I think that he will shake it off," Olsen said afterwards.
Rooney, 32, signed a multiyear deal with United in late June after transferring from Everton. He debuted on July 14 against the Vancouver Whitecaps, entering the match in the second half.