The Cubs open the 2014 campaign on March 31 against the Pittsburgh Pirates.
Samardzija also started last season's Opening Day, giving up two hits and striking out nine in a 3-1 victory over the Pirates.
"Jeff will take the nod," Renteria said via MLB.com. "Everybody's looking forward to him taking the hill against Pittsburgh."
Samardzija went 8-13 with a 4.34 ERA, 1.35 WHIP, and 214 strikeouts for the Cubs last season. Last month, he signed a one-year, $5.345 million contract for 2014, avoiding arbitration. Renteria said lefthander Travis Wood will follow Samardzija in the rotation.
"We'll go Woody [in the second game] and keep working on the rest," Renteria said of the team's rotation. "[Edwin Jackson] should follow, and everybody will follow in line." "Jeff's done it before and put in a very strong year, as Woody did," Renteria said. "I think the decision was quite easy for all of us."