McCutchen was placed on the 15-day DL on Aug. 11, retroactive to Aug. 4, with a rib injury. Tuesday is the first day the 27-year-old is eligible to be activated.
The outfielder last played on Aug. 3, when he was forced to exit a game against the Arizona Diamondbacks after experiencing discomfort in his left side.
The reigning National League MVP, McCutchen was hitting .311 with a .947 OPS and 17 home runs before the injury.
The Pirates entered Monday at 64-60, 1.5 games behind the San Francisco Giants for the second NL Wild Card.
- Ben Estes