The two-time All-Star is hitting .260 with three home runs, nine RBIs and a .716 OPS in 40 games this season. He was injured sliding into second base on a steal attempt.
Cole Figueroa was optioned to Triple-A Durham to make room for Zobrist on the 25-man roster.
Zobrist has played his entire nine-year career with the Rays, hitting 107 home runs with 468 RBIs.
Tampa Bay (23-31) is off on Thursday and is currently in last place in the AL East, nine games behind Toronto.