Both players are dealing with groin injuries, and Jackson was limited in practice throughout the week. Coach Doug Marrone said Friday that Jackson currently is not ready to play.
Watkins left practice Wednesday after aggravating the injury. Watkins revealed Friday that he's been dealing with the groin injury for two weeks.
Jackson was injured against Minnesota on Oct. 19. He has 239 yards and one touchdown on 55 carries this season. The Bills are already without running back C.J. Spiller (broken collarbone).
Watkins has 38 receptions for 590 yards and five touchdowns.