Gwen Jorgensen's 13-race winning streak was snapped as Helen Jenkins of Great Britain took the victory in 1:56:03 at the ITU World Triathlon Series' Gold Coast race.
Jorgensen crossed the finish line 41 seconds later settled for silver. Jorgensen tried to catch up at the end of the race and recorded the fastest run in 34:17 but came up short. It marks her first major loss since finishing third at the WTS in Capetown on April 26, 2014.
The former Ernst and Young accountant is considered an early favorite for the gold medal at the 2016 Olympics in Rio de Janeiro.
New Zealand's Andrea Hewitt took bronze in Saturday's race.
Jorgensen won the ITU World Cup in New Zealand last week. She finished last season as the world triathlon champion and won seven WTS races.
The United States will be represented by Jorgensen and Sarah True in Rio de Janeiro as one spot remains open. True was fourth in London as Jorgensen finished way back in 38th place after suffering a flat tire on the bike ride.
Katie Zafares, who is being considered for that third Olympic spot, finished sixth overall in 1:58:14 and was the second American overall.
The World Triathlon Series continues on April 24 in Capetown, South Africa.
- Chris Chavez