Reyes has been bothered by soreness in his left side for several days, and said the injury affects him most when swinging from the left side.
Batting left-handed against Rays right-hander Chris Archer, Reyes grimaced and held his side after grounding out in the first inning. Once back in the dugout, h slammed his batting helmet to the ground and disappeared up the tunnel to the clubhouse.
Ryan Goins, who was recalled from Triple-A Buffalo earlier in the day, replaced Reyes in the second.
Blue Jays manager John Gibbons said before the game that he intended to give Reyes a day off Saturday, with Steve Tolleson expected to start in his place.
Gibbons said the decision to recall Goins was not made because of Reyes' lingering pain, but to get Toronto back to a seven-man bullpen. Goins replaces right-hander Todd Redmond, who was designated for assignment.