Charles left Sunday's Week 2 loss to the Broncos with a high ankle sprain. He had just two carries for 12 yards before leaving the game during the first quarter.
In 15 games last season, Charles had 1,287 rushing yards and a league-leading 12 touchdowns for the Chiefs.
With Charles out of the lineup, Knile Davis had 22 carries for 79 yards against the Broncos.
The Chiefs are 0-2 and have lost nine of their past 11 games after a 9-0 start last season. They will play the Dolphins in Miami on Sunday.
- Paul Palladino