The South Side squad will instead compete in the Babe Ruth League.
After being stripped of its national title in February, Chicago-based Jackie Robinson West has decided to split from Little League, according to the Chicago Tribune.
The team will now compete in the Babe Ruth League, a team spokesman told the Tribune.
Jackie Robinson West gained national acclaim during its run to the Little League World Series last summer. The club won the United States title before falling in the Little League World Series championship game to South Korea.
In February, Jackie Robinson West vacated its national title after a Little League investigation found the team had knowingly violated residency rules.
Jackie Robinson West said it was separating from Little League due to the "disrespect" the organization showed the team.
"It's largely based on the disregard and disrespect shown by Little League that had a 43-year-old relationship with Jackie Robinson West," spokesman Glenn Harston told the Chicago Tribune.
The spokesman told the Tribune that moving to Babe Ruth would allow the team to remain together.
- Stanley Kay