Bayne turned the fast lap during qualifying at Daytona International Speedway on Friday, averaging 177.162 mph around the 2 1/2-mile superspeedway.
Bayne is driving the No. 6 Ford for Roush Fenway Racing, the car Ricky Stenhouse Jr. piloted to the last two championships in the second-tier series.
Sam Hornish Jr. will start second, followed by Parker Kligerman, Travis Pastrana and Austin Dillon.
Full-time Sprint Cup Series drivers Kyle Busch (seventh), Matt Kenseth (ninth), Tony Stewart (10th), Brad Keselowski (11th), Danica Patrick (12th), Dale Earnhardt Jr. (13th) and Kasey Kahne (19th) also are entered in Saturday's 300-mile race.
Kyle Larson, a rising star in NASCAR's ranks, will start 21st in his Nationwide debut.