Divine Myles added 13 points, on 6-of-9 shooting, and Angel Rivera had seven assists for Stetson (3-5), which made 24 of 47 field goals (51.1 percent), including 8 of 19 (42.1 percent) from 3-point range.
Stetson, which had 17 assists, took a 32-24 lead into halftime and held off Florida A&M (0-7) for the win.
Jorge Rosa led the Rattlers with a career-high 21 points and Jermaine Ruttley added 10 points, eight rebounds and four assists.
Florida A&M struggled shooting the ball, hitting just 19 of 50 from the floor, 3 of 14 from behind the arc and 9 of 16 free throws.
Stetson out-rebounded the Rattlers 38-30.